Re: Image transform

Posted by Gabriel Landini on
URL: http://imagej.273.s1.nabble.com/Image-transform-tp3703413p3703422.html

On Friday 03 March 2006 16:25, Liu, Dongfang (NIH/NIAID) [E] wrote:
> Thank you for your help.
> Yes, the system is a miroscope. Could you tell what other optics I should
> consider to calibrate a image?

No, I couldn't because I do not know what kind of optics your microscope has.

You have to do this:

> > You need to put a known
> > sized object under your imaging system and see how many pixels it spans.
> > Then you estimate the size of your pixels dividing the known size of your
> > object by the number of pixels it occupies.

Cheers,

Gabriel