Posted by
Guenter Giese on
URL: http://imagej.273.s1.nabble.com/image-intensity-correction-tp3703695p3703699.html
Hi all:
a caveat: the plugin mentioned corrects for background (only). If your
brightness values (signal plus background) are lower in the edges, this may
be caused by the optical design of the instrument (illumination diminution
at the edges plus detection diminution, also known as vignetting in
photography). In this case you would have to apply some kind of
position-dependent correction implying multiplication or division, not
position-dependent offset correction.
Is there a plugin doing that?
Guenter
> -----Original Message-----
> From: ImageJ Interest Group [mailto:
[hidden email]] On
> Behalf Of marefat
> Sent: Samstag, 11. Februar 2006 00:16
> To:
[hidden email]
> Subject: Re: image intensity correction
>
> Hi Elena:
>
> Thank you very much. It is excellent.
>
> Thank you,
> Yousef
>
> On 2/10/06, Elena Kardash <
[hidden email]> wrote:
> > Hi, Joe,
> > try this: Process/Subtract Background
> >
> >
http://rsb.info.nih.gov/ij/docs/menus/process.html#background> > the plugin is described here:
> >
http://rsb.info.nih.gov/ij/plugins/background.html> >
> > it works really nice
> >
> > Elena
> >
> >
> > marefat wrote:
> >
> > >Hi -
> > >
> > >Is there a way to correct for signal non-uniformity across
> an image.
> > >
> > >Thanks
> > >
> > >Joe
> > >
> > >
> >
>