http://imagej.273.s1.nabble.com/X-Y-coordinates-to-pixels-tp3703825p3703826.html
I have the same problem. Could I calculate the displacement of two particles according to the pixel size?
> ----------
> From: Curtis Rueden
> Reply To: List IMAGEJ
> Sent: Wednesday, February 1, 2006 3:20 PM
> To: List IMAGEJ
> Subject: Re: X-Y coordinates to pixels
>
> Hi Leslie,
>
> I do not know if there is a way to measure points at higher precision in
> ImageJ (though I would be surprised if there is not), but this is one of
> the things VisBio (
http://www.loci.wisc.edu/visbio/) can do. You can
> place markers in double-precision floating point, and then export them
> to a text file for use in a spreadsheet. See the Overlays page in the
> "Data Transforms" section of the program's built-in help for details.
>
> -Curtis
>
> Leslie Day wrote:
>
> >I am hoping someone could help me. I am trying to map a point as it moves
> >through a series of images in order to find out velocity of the object and
> >distance traveled.. I figured the best way would be to keep creating points
> >with the point selector and then map the points. However, currently when I
> >use the point selector it gives me x-y coordinates in whole numbers. I need
> >something with more significant figures (3 decimal spots) to be able to see
> >the movement. Maybe in pixels or ?m. Perhaps there is another way to detect
> >linear velocity and distance traveled through a series of images.
> >
> >
> >
> >Thanks
> >
> >
> >
> >Leslie Day
> >
> >Biology Department
> >
> >Northeastern University
> >
> >
[hidden email]
> >
> >617-373-3780
> >
> >
>