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Re: X-Y coordinates to pixels

Posted by Liu, Dongfang (NIH/NIAID) [F] on Feb 02, 2006; 2:41pm
URL: http://imagej.273.s1.nabble.com/X-Y-coordinates-to-pixels-tp3703825p3703830.html

Dear Sir,

Could you do it in Image Pro or Image J? How to do 2-Dimension Gauss Fit(for a fluorescence spot)?

> ----------
> From: Michael Cammer
> Reply To: List IMAGEJ
> Sent: Thursday, February 2, 2006 9:36 AM
> To: List IMAGEJ
> Subject: Re: X-Y coordinates to pixels
>
> To measure particles, either you need to do it per pixel which, by
> definition, is a discrete number, or you need to look at a block of pixels
> and do a gaussian or other fit if you need to find a fraction of a pixel
> location.
> -mc
>
>
>
> At 09:14 AM 02/02/06 -0500, you wrote:
> >Dear Sir,
> >
> >I have the same problem. Could I calculate the displacement of two
> >particles according to the pixel size?
> >
> >
> >
> >
> >
> >
> >
> >**********************************************
> >Dongfang Liu
> >Laboratory of Immunogenetics
> >National Institute of Allergy and Infectious Disease
> >National Institute of Health
> >Twinbrook II, Room 207
> >12441 Parklawn Drive
> >Rockville, MD 20852
> >Phone: 301-496-2951
> >Fax:301-402-0259
> >email:[hidden email]
> >**********************************************
> >
> > > ----------
> > > From:         Curtis Rueden
> > > Reply To:     List IMAGEJ
> > > Sent:         Wednesday, February 1, 2006 3:20 PM
> > > To:   List IMAGEJ
> > > Subject:      Re: X-Y coordinates to pixels
> > >
> > > Hi Leslie,
> > >
> > > I do not know if there is a way to measure points at higher precision in
> > > ImageJ (though I would be surprised if there is not), but this is one of
> > > the things VisBio (http://www.loci.wisc.edu/visbio/) can do. You can
> > > place markers in double-precision floating point, and then export them
> > > to a text file for use in a spreadsheet. See the Overlays page in the
> > > "Data Transforms" section of the program's built-in help for details.
> > >
> > > -Curtis
> > >
> > > Leslie Day wrote:
> > >
> > > >I am hoping someone could help me. I am trying to map a point as it moves
> > > >through a series of images in order to find out velocity of the object and
> > > >distance traveled.. I figured the best way would be to keep creating
> > points
> > > >with the point selector and then map the points. However, currently when I
> > > >use the point selector it gives me x-y coordinates in whole numbers. I
> > need
> > > >something with more significant figures (3 decimal spots) to be able
> > to see
> > > >the movement. Maybe in pixels or ?m. Perhaps there is another way to
> > detect
> > > >linear velocity and distance traveled through a series of images.
> > > >
> > > >
> > > >
> > > >Thanks
> > > >
> > > >
> > > >
> > > >Leslie Day
> > > >
> > > >Biology Department
> > > >
> > > >Northeastern University
> > > >
> > > >[hidden email]
> > > >
> > > >617-373-3780
> > > >
> > > >
> > >
>
> ____________________________________________________________________________
> Michael Cammer   Analytical Imaging Facility   Albert Einstein Coll. of Med.
> Jack & Pearl Resnick Campus      1300 Morris Park Ave.     Bronx, NY  10461
> (718) 430-2890       Fax:  430-8996      URL:  http://www.aecom.yu.edu/aif/
>    **This electronic transmission contains information that is privileged.**
>
>