http://imagej.273.s1.nabble.com/X-Y-coordinates-to-pixels-tp3703837p3703838.html
coordinates or coordinates in units specified in set scale dialog. If
scale parameter. Then you'll get smaller pixels (in scaled unit) and
more precision with your measurements. However, resampling can
structures when their size is close to the size of single pixels. So
Le 3 févr. 06 à 15:12, Leslie Day a écrit :
> Okay. Then I rephrase my question... How can I get image J to give me
> another measurement of a point, besides pixels. Some that is much more
> precise and can help me measure small amounts of movement?
>
> I am not sure what a Gaussian is.
>
> Leslie Day
> Biology Department
> Northeastern University
>
[hidden email]
> 617-373-3780
>
> -----Original Message-----
> From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of
> Michael Cammer
> Sent: Thursday, February 02, 2006 9:37 AM
> To:
[hidden email]
> Subject: Re: X-Y coordinates to pixels
>
> To measure particles, either you need to do it per pixel which, by
> definition, is a discrete number, or you need to look at a block of
> pixels
> and do a gaussian or other fit if you need to find a fraction of a
> pixel
> location.
> -mc
>
>
>
> At 09:14 AM 02/02/06 -0500, you wrote:
>> Dear Sir,
>>
>> I have the same problem. Could I calculate the displacement of two
>> particles according to the pixel size?
>>
>>
>>
>>
>>
>>
>>
>> **********************************************
>> Dongfang Liu
>> Laboratory of Immunogenetics
>> National Institute of Allergy and Infectious Disease
>> National Institute of Health
>> Twinbrook II, Room 207
>> 12441 Parklawn Drive
>> Rockville, MD 20852
>> Phone: 301-496-2951
>> Fax:301-402-0259
>> email:
[hidden email]
>> **********************************************
>>
>>> ----------
>>> From: Curtis Rueden
>>> Reply To: List IMAGEJ
>>> Sent: Wednesday, February 1, 2006 3:20 PM
>>> To: List IMAGEJ
>>> Subject: Re: X-Y coordinates to pixels
>>>
>>> Hi Leslie,
>>>
>>> I do not know if there is a way to measure points at higher
>>> precision in
>>> ImageJ (though I would be surprised if there is not), but this is
>>> one of
>>> the things VisBio (
http://www.loci.wisc.edu/visbio/) can do. You can
>>> place markers in double-precision floating point, and then export
>>> them
>>> to a text file for use in a spreadsheet. See the Overlays page in
>>> the
>>> "Data Transforms" section of the program's built-in help for
>>> details.
>>>
>>> -Curtis
>>>
>>> Leslie Day wrote:
>>>
>>>> I am hoping someone could help me. I am trying to map a point as it
> moves
>>>> through a series of images in order to find out velocity of the
>>>> object
> and
>>>> distance traveled.. I figured the best way would be to keep
>>>> creating
>> points
>>>> with the point selector and then map the points. However, currently
> when I
>>>> use the point selector it gives me x-y coordinates in whole
>>>> numbers. I
>> need
>>>> something with more significant figures (3 decimal spots) to be
>>>> able
>> to see
>>>> the movement. Maybe in pixels or ?m. Perhaps there is another
>>>> way to
>> detect
>>>> linear velocity and distance traveled through a series of images.
>>>>
>>>>
>>>>
>>>> Thanks
>>>>
>>>>
>>>>
>>>> Leslie Day
>>>>
>>>> Biology Department
>>>>
>>>> Northeastern University
>>>>
>>>>
[hidden email]
>>>>
>>>> 617-373-3780
>>>>
>>>>
>>>
>
> ______________________________________________________________________
> ______
> Michael Cammer Analytical Imaging Facility Albert Einstein
> Coll. of Med.
> Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx,
> NY 10461
> (718) 430-2890 Fax: 430-8996 URL: http://
> www.aecom.yu.edu/aif/
> **This electronic transmission contains information that is
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