Spematozoid segmentation

Posted by Juanma Pascual Gaspar on Feb 02, 2006; 2:56pm
URL: http://imagej.273.s1.nabble.com/Spematozoid-segmentation-tp3703843.html

Hello, I am developing a tool for counting spermatozoids based on the ImageJ
ParticleAnalizer plugin. The thing is the PA plugin works well with a very
defined binary image, but in the binary segmentation process (based on pixel
filtering wihin a range of gray scale levels) I can't diferentiate between
genuine spermatozoid and another cells with the same size and gray scale
intensity.

The difference between these cells and the indesired cells
(denominated round cells) is the absence of tail. Does anybody knows how I
can do this with (preferably with a ImageJ plugin or similar).

Many thanks in advance.
 Juan