Re: Spematozoid segmentation

Posted by jiho on Feb 02, 2006; 3:46pm
URL: http://imagej.273.s1.nabble.com/Spematozoid-segmentation-tp3703843p3703844.html

On 2 févr. 06, at 15:56, Juanma Pascual Gaspar wrote:

> Hello, I am developing a tool for counting spermatozoids based on  
> the ImageJ
> ParticleAnalizer plugin. The thing is the PA plugin works well with  
> a very
> defined binary image, but in the binary segmentation process (based  
> on pixel
> filtering wihin a range of gray scale levels) I can't diferentiate  
> between
> genuine spermatozoid and another cells with the same size and gray  
> scale
> intensity.
>
> The difference between these cells and the indesired cells
> (denominated round cells) is the absence of tail. Does anybody  
> knows how I
> can do this with (preferably with a ImageJ plugin or similar).

if you see the tail in the binary image you could filter your results  
based on the ratio circumference/area of the particles. The round  
ones will probably have a much smaller circumference than the  
spermatozoids, for a the same area. It will require some calibration  
to find the good range of values but the analyze particle function  
can give you both the area and the circumference of particles with no  
problem.

> Many thanks in advance.
>  Juan

JiHO
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