Re: Custom build bacterial colony counter hardware.

Posted by Olivier Salvado on Jan 23, 2006; 2:09pm
URL: http://imagej.273.s1.nabble.com/Re-Custom-build-bacterial-colony-counter-hardware-tp3703971p3703974.html

We don't use a particular plug-in, just regular operations and a script in
Matlab for batch processing.

Olivier Salvado

 
-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Duleep
Samuel
Sent: Sunday, January 22, 2006 10:51 PM
To: [hidden email]
Subject: Re: Custom build bacterial colony counter hardware.

I would like to receive a copy of the plugin regards Samuel

On 1/23/06, Olivier Salvado <[hidden email]> wrote:

>
> We got around the same problem although for a different setup by shooting
> at
> each session a calibration phantom.
>
> Briefly:
> We shoot a white paper (you don't want a specular material) that we put in
> place of the sample.
> We tune the exposure and the white balance of the camera on the white
> phantom.
> Each subsequent image is corrected for background illumination using the
> white paper.
> We found the method simple yet very powerful.
>
> White balance and exposure can be performed by image processing if not
> easily feasible on the camera itself.
>
> Olivier Salvado
>
>
> -----Original Message-----
> From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
> Stella
> Sim
> Sent: Sunday, January 22, 2006 4:58 PM
> To: [hidden email]
> Subject: Re : Custom build bacterial colony counter hardware.
>
> Dear lists,
>
> I think my question is irrelevant to the list, but I thought I ask anyway,
> as i don't know who to turn to.
>
> I have written a plugin performing automated bacterial colony counts
> (Streptococus Uberis) using ImageJ (accredited to ImageJ's user list).
> However, I constantly encountered image's quality issues like uneven
> shadow
> from the rim of the dish, levelness of the dish divider and constrast. The
> tools used to photograph the dish are very primitive( a digital camera,
> lightbox and tripod). The lab technician set up the equipments in every
> photograph session, and pack them away after finish. The variations in
> every photograph session were visible in the images (despite  in their
> best
> effort to keep everything consistent). There is no control of the natural
> lighting at all. I want to build them a custom lightbox with a camera
> moulded on top ... Have anyone attempt that ? Any suggestions are welcome
> !
> FYI, Purschasing a colony counter hardware is out of question.
>
> Thanks in advance.
>
> Stella
>