Re: Ratio and Background subtraction operations

Posted by Michael Cammer on Jan 18, 2006; 12:12pm
URL: http://imagej.273.s1.nabble.com/Ratio-and-Background-substraction-operations-tp3704031p3704035.html

Everything is for the best in the best of all possible worlds?
Hey, we're just talking intensities; try measuring lifetimes!

> Hello,
> Just as a remark, in biology applied microscopy, fluorescence is not so
> proportional to the number of molecules. One knows that environment of
> the dye could have a dramatic inluence on quantum efficiency (presence
> of quenchers, pH differences inducing spectral modifications,... in the
> different cell compartments).
> I agree for all the other considerations ;-)