Collecting PSF files for Zeiss 100X oil objective

Posted by Krishanu Ray on Dec 15, 2005; 2:09pm
URL: http://imagej.273.s1.nabble.com/Collecting-PSF-files-for-Zeiss-100X-oil-objective-tp3704216.html

Hello every one,

We are trying to use the deconvolutionJ plug-in to deconvolve an
epifluorescent image stack, collected using the Zeiss 100X NA 1.4
NeoFluar objective and a Hamamatsu ORCA camera.

Now we are wondering how to make the PSF file for the objective,
which is esssential for the deconvolution.

We will be grateful if someone could help us with a setp-by-step
instruction, or, it would be even better if someone could supply the file.

many thanks,

krishanu.

Krishanu Ray
Department of Biological Sciences
Tata Institute of Fundamental Research
Homi Bhabha Road, Colaba, Mumbai 400 005.
India.
Ph: [phone] ext 2730
Fax: [phone]