Analyze particle & Flood fill

Posted by Corentin Cras-Méneur-2 on Dec 09, 2005; 4:56pm
URL: http://imagej.273.s1.nabble.com/Analyze-particle-Flood-fill-tp3704248.html

        Hi All,


    My apologies if the question have been already raised on the list,
but I search for a while and did not find any mention of it.
    I've been using the Analyze Particle function to measure tissue
areas on calibrated TIFF images and I noticed two things that threw
me off for quite a while:

- the Flood Fill option seems to work the opposite way as what I
would have anticipated (or I simply did not understand it): when the
option is checked, the measurements are performed *without* filling
the inner-spaces and when the Flood Fill option is unchecked, the
measurements include these areas.

- Setting the maximal size appears to be critical. If I don't set
anything, the largest elements on the thresholded image will simply
be ignored (as can be visualized with the masks). If I set a value
that's too big (I would say, larger than the entire area in the
image), then I have the same problem. I have to set it to a value
larger than these objects (obviously) but also smaller than the
surface of the entire image.
I would have thought that not setting the max value would have been OK.

Are these known issues? Is there something I don't understand??
Thanks,


                Corentin