Colocalisation analysis

Posted by Lee Kong Heng on Nov 15, 2005; 9:40am
URL: http://imagej.273.s1.nabble.com/Colocalisation-analysis-tp3704464.html

Hi Folks,

  I think I'm doing something wrong as the scatter plot should filled
the whole graph instead just part of it:

http://img383.imageshack.us/my.php?image=funnyscatterplot2lm.jpg

Here's what I did:

1. Open all files 24 bit TIFF (Green only and Red only fluorescence
images).

2. Convert to 8 bit for each image: Image>Type>8 bit

3. Analysis: Plugins>Colocalisation analysis>Manders coefficients

4. Manders Calculator appears:
  Set green image to green channel, red to red channel
  None for use ROI
  Selected Display Colour Scatterplot
  Selected Exclude zero-zero pixels

5. Press ok. Statistics seems fine but scatterplot is funny.

  I'll be grateful for any advice that you may share and thank you.

Sincerely,
Kong Heng