Posted by
Lee Kong Heng on
Nov 16, 2005; 7:57am
URL: http://imagej.273.s1.nabble.com/Colocalisation-analysis-tp3704464p3704465.html
Hi Guenter,
It worked. Thank you very much.
Sincerely,
Kong Heng
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Guenter Giese
Sent: Tuesday, November 15, 2005 6:24 PM
To:
[hidden email]
Subject: AW: Colocalisation analysis
Hi Kong,
I think this is caused by the type of conversion you use.
First split the 24bit RGB images into their channels and then use the respective green or red channel for your analysis. Otherwise you reduce your brightness (intensity) values due to the conversion process.
Hope this helps
Guenter
------------------------------------------
Dr. Guenter Giese
Light Microscopy Facility Manager
Dept. of Biomedical Optics, MPI fuer Medizinische Forschung Jahnstr. 29, D-69120 Heidelberg, Germany Phone (+49) 6221-486-360 (Fax: -325)
e-mail:
[hidden email]
http://lightmicro.mpimf-heidelberg.mpg.de-----Ursprüngliche Nachricht-----
Von: ImageJ Interest Group [mailto:
[hidden email]] Im Auftrag von Lee Kong Heng
Gesendet: Tuesday, 15 November, 2005 10:40
An:
[hidden email]
Betreff: Colocalisation analysis