Grid for volume density calculation

Posted by Vivek Balasubramaniam on Nov 09, 2005; 5:28am
URL: http://imagej.273.s1.nabble.com/Grid-for-volume-density-calculation-tp3704500.html

I am looking for some assistance. we are trying to analyze lung  
slices with a aim to estimate the ratio of the volume density of the  
lung as compared to the airspaces. I would like to overlay a grid of  
crosshairs over an image of the lung and be able to count the number  
of times the grid intersects the lung and the airspaces. We used to  
use a program called Stereology Toolbox for the Mac OS9 to do this  
but would like to use ImageJ so that it could be used cross platform  
and on TIFF images. I am hoping that there is a plug in already  
created out there that can do this, before having to create one.

The problem is that we are dealing with  very small lungs and  
sometimes we cannot fill the entire field of view with the lung slice  
and would like to do a volume density calculation as described in  
Howard and Reed.

thanks in advance,

vivek

Vivek Balasubramaniam, MD
Assistant Professor
Director, Pediatric Heart Lung Center Laboratory
University of Colorado Health Sciences Center
The Children's Hospital
(clinical office) 303-837-2522
(lab) 303-724-4062
(pager) 303-826-8560