Measuring moving cells
Posted by Luciano Marpegan on Nov 07, 2005; 4:00pm
URL: http://imagej.273.s1.nabble.com/Measuring-moving-cells-tp3704522.html
Hi All,
I am working with stacks of fluorescent images with many cells in
each frame. I want to measure the gray value (mean) of each cell in
each frame but some of them move from frame to frame. Multimeasure
plugin works ok if the cells don't move too much but if they move
outside the original Roi I can't measure them any more. An option
would be to use the manual tracking plugin but I couldn't manage to
get the gray values of circular Rois with it. Analyzing cell by cell
in each frame is really time consuming and a really hard task since
we have long stacks with too many cells. I don't want to reinvent the
wheel (I don't even think I can do it) and I guess someone probably
got around this problem before. So any insights on this issue will be
greatly appreciated.
Best,
Luciano