Re: Frequency and area analysis

Posted by Pedro Alejandro Herve Fernandez Carrera Licenciatura en Cs.Biologicas on Oct 13, 2005; 1:53am
URL: http://imagej.273.s1.nabble.com/Frequency-and-area-analysis-tp3704675p3704679.html

hi, if the cells are beating or moving (quite impressive), and you are also
taking a stack of pictures (five per second), open the sequence (file/open as
image sequence), then go to edit and invert them (all the pictures will be
black and white), make a copy of the first picture of the stack (by pressing
shift + control + d), now you make a selection of your culture and copy (by
pressing "c"), move it over the cell or cells that beats, and press "v",
after all this go to process/image calculator and substract the copy to the
stack (process all the stack), then go to image/adjust threshold to make your
cells visible, and then i think you can use an area measurer plugin (if you
find it please contact me).
this example i took ot from the moving analisys of two larvae on the web page
of imagej.
sorry about my english.
let me know if it worked.
bye