background subtraction update...sort of..

Posted by sean_incali on Sep 28, 2005; 3:31am
URL: http://imagej.273.s1.nabble.com/background-subtraction-update-sort-of-tp3704770.html

I have reinstalled IMagej and upgraded to the newest version.

Bacgkround subtraction now works in green channel but not in blue. (using 50 as
radius and white background option unchecked)

<a href="http://imageshack.us"><img
src="http://img399.imageshack.us/img399/6706/t1rgb0tv.png" border="0"
width="512" alt="Image Hosted by ImageShack.us" /></a>

shows the image used. (i uploaded the tiff, but somhows it became png. )

Open the tiff in IMAGEJ Imgae> Color> RGBsplit> then take a historgam before
and after the background subtraction.

Upon examining the histogram, you will see that Green channel mean is lower
after background subtraction. But blue channel mean is identical.

Am I not doing this right?