Re: background removal in fluorescence

Posted by Joel Sheffield on Sep 20, 2005; 3:29pm
URL: http://imagej.273.s1.nabble.com/background-removal-in-fluorescence-tp3704817p3704822.html

I would imagine that part of the problem is that you are trying to
remove background signal which is mostly dark.  This means that the
pixel values are close to zero or so.  Image subtraction might cause
the values to drop below zero, which wouldn't be helpful.  Some times
I have found it useful to divide the data image by the background
image, using a 16 or 32 bit resultant image, and then readjust.    

Joel

> Dear ImageJ folks--
>
> Has anyone come up with a good method for background subtraction in
> images obtained using fluorescence microscopy?  In my hands, the
> methods that work for brightfield don't work for fluorescence.
>
> Thanks!
>
> Martin
> --
> Martin Wessendorf, Ph.D.                   office: [phone]
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Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
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