Re: background removal in fluorescence

Posted by Pedro J CamelloDr Pedro J Camello on Sep 19, 2005; 11:53pm
URL: http://imagej.273.s1.nabble.com/background-removal-in-fluorescence-tp3704817p3704823.html

In my opinion (my main experience is ca2+ signals in living cells) the
best method is to acquire a background image in an area of the coverslip
where there are no cells, using it to substract to the series of images.
Sometimes this is difficult if the preparation is too "dense", and if
you have to move too far from the area of interest the plane of focus
could be different. Now I´m removing to the entire image the average
fluorescence from an irregular ROI drawn in the "empty" area surrounding
the cells.

Martin Wessendorf wrote:

> Dear ImageJ folks--
>
> Has anyone come up with a good method for background subtraction in
> images obtained using fluorescence microscopy?  In my hands, the
> methods that work for brightfield don't work for fluorescence.
>
> Thanks!
>
> Martin