analysis of objects with holes

Posted by luis amaral on Sep 14, 2005; 6:19pm
URL: http://imagej.273.s1.nabble.com/analysis-of-objects-with-holes-tp3704830.html

Hello.
I am trying to determine the number of pixels (or length) of the
skeleton of the objects within a given image. Some of the objects
however have holes inside leading to skeletons like a circle. When I try
to determine the area of these skeletons by the macro command
run("Analyze Particles...",…) this command always fill the holes within
the circle and determines the filled area. How can I prevent this? Or is
there another way to determine each skeleton individual length?
I would very much appreciate if someone could help me.
Luís Amaral
 
__________________________________________________________________
­­­­­­­­­­­­
António Luís Pereira do Amaral
Departamento de Tecnologia Química
Escola Superior de Tecnologia e de Gestão - Instituto Politécnico de
Bragança
Campus de Santa Apolónia, 5301-857 Bragança – Portugal
Tel.: [phone]
Tlm.: ([phone]
e-mail:  <mailto:[hidden email]> [hidden email];
<mailto:[hidden email]> [hidden email]
 <http://www.deb.uminho.pt/BioPSEg/lpamaral>
http://www.deb.uminho.pt/BioPSEg/lpamaral
 <http://dtq.estig.ipb.pt> http://dtq.estig.ipb.pt
__________________________________________________________________