Re: what is the reason for the difference seen here

Posted by sean_incali on
URL: http://imagej.273.s1.nabble.com/what-is-the-reason-for-the-difference-seen-here-tp3704870p3704872.html

> ImageJ does automatic contrast/brightness adjustment for display under
> some circumstances.  If you examine the histogram for the image, you'll
> find that it's unaltered.
>
http://img369.imageshack.us/img369/3179/compare6cb.jpg 


How do you use the histogram to check? Are you comparing the intensity
distributions? (I guess that's the only thing I can think of using Histogram to
check...)

Also I have a question about opening a lsm file using LSM plugin vs using an
image in Tiff format exported from LSM broswer.

If I open the "Tiff file" in ImageJ, is that same as the lsm file opened using
the plugin? (same, I mean in terms of intensity in two channels.)

If they are different, how different are they? in what respect?