Help with moving objects

Posted by Pedro J CamelloDr Pedro J Camello on Sep 09, 2005; 2:20am
URL: http://imagej.273.s1.nabble.com/Help-with-moving-objects-tp3704891.html

Hi all,

I´m measuring fluorescence from living cells while they are perfused in
a microscope. The fluid and the applied treatment causes frequent
movement of the cells (smooth muscle). I need to make plot of the
fluorescence in the "cellula" ROI along hundreds of images per
experiment, so that re-drawing the rois when the cells "escape" from the
ROI would be too slow and tedious.

I would like to know how to perform whether the following approach (and
if you think it could work!):

1- I draw a rectangular ROI around each cell, big enough that the cell
doesn´t "escapes" when moves or contracts
2- I define a minimum threshold to create a mask. This mask would be
choosen as the area where measurement (area and average value) would be
performed
3- This must be done with several ROIs/cells in a stack, and within each
ROI the pixels above the threshold would give a Z proyection

Even small help would be very useful :)
Thanks in advance