Posted by
Mark McNabb on
Aug 23, 2005; 7:41pm
URL: http://imagej.273.s1.nabble.com/AnalySIS-TIFF-images-tp3704983p3704985.html
Hi Eric,
This is a good reference on TIFF metadata:
http://www.awaresystems.be/imaging/tiff/tifftags/baseline.htmlI don't know if there is a way to extract the information you need from
the TIFF file using ImageJ, others may know. I think that some of these
systems can store this data in propietary formats or though history data
and then only export TIFFs without resolution unit data. Sometimes the
best bet is the use the function in the camera software to place a scale
bar on the image -- it can be hard to figure out as I have we have this
same camera on our TEM and have heard others complain about it. I'm just
using negatives still on that 'scope.
Mark.
On Tue, 23 Aug 2005, Eric LEROY wrote:
> Hi,
>
> I work on a FEI tecnai F20 electron microscope and we have a SIS
> MegaView III camera installed. The software used for the acquisition is
> analysis from SIS. This software produces 16 or 8 bits TIFF images that are
> readable by ImageJ. But the spatial calibration that should written inside
> the file is not read by ImageJ and therefore the images are not calibrated
> under ImageJ. Does anybody have an idea were and how the spatial calibration
> is stored in these images?
>
> Thank you
>
> Best regards
>
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