Re: reformatting RGB stack

Posted by Wayne Rasband on Aug 18, 2005; 6:11pm
URL: http://imagej.273.s1.nabble.com/Re-Problems-loading-image-from-streaming-servlet-tp3705002p3705007.html

> I am working with large (1380x1024x450) stacks of
>  RGB (24bits) images from episcopic microscope. I
>  would like to reformat my dataset at an arbitrary
> angle (reformatting the whole volume). All I found
>  seemed to work only for gray level images.
>
>  I thought about splitting the three RGB planes,
>  reformatting each one separately and reforming a
>  RGB stack. This sounds like trouble, Any better
>  suggestion?

The Rotate plugin that is part of the TransformJ package at

     http://www.imagescience.org/meijering/software/transformj/

can rotate a stack an arbitrary number of degrees around any of its
axes.

You will probably need to reduce the size of the images when importing
them using File>Import>Image Sequence. A 1380x1024x450 RGB stack
requires 2.4 GB of memory. Setting "Scale Images" in the
File>Import>Image Sequence dialog to 50% reduces this to 606 MB.

-wayne