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Image Analysis using Image J

Posted by Sharat Pankajavihar Sasi on Aug 18, 2005; 6:30pm
URL: http://imagej.273.s1.nabble.com/Image-Analysis-using-Image-J-tp3705013.html

Hello,
       I am a new user of the Image J software and had few queries on the
application I am looking for. My issue is as stated below:

"I have Calibration pictures that were taken at 40X lense and with different
zooms on the camera say 50, 77, 88 etc. These images taken need to be
calibrated from pixels to microns in the Image J software if possible. Then,
the stained images of the tissue taken with the same lens and zoom under the
same miscroscope need to be added in the software and the thickness and
diameter need to be measured in microns."

Can this be done with Scion imaging software? Could you please advice me on the
same? If its not possible can anyone please suggest me an alternative?

Thanking You,

Sharath. P. Sasi

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