Re: Image Analysis using Image J

Posted by Sharat Pankajavihar Sasi on Aug 18, 2005; 8:18pm
URL: http://imagej.273.s1.nabble.com/Image-Analysis-using-Image-J-tp3705013p3705015.html

Hi Mark,
           Thanks a lot for your reply. I will do as you say and try out using
Scion Image. If I have any more queries I will get back with you.

Thanks a lot Once again!!!

Sharath

Quoting Mark David McNabb <[hidden email]>:

> On Thu, 18 Aug 2005, Mark David McNabb wrote:
>
> Hi Sharat,
>
> I have done this in both ImageJ, NIH Image, and Scion Image.  It is
> relatively straight forward if you do it manually.  You would just
> use the calibration images - drawing straight segmented lines across
> your known calibration grid or sphere distance - and the "Analyze ->
> Set Scale" menu to calculate the calibration numbers for each zoom
> setting.  Then when you open an image at a certain zoom and
> magnification, you would just go to that same menu item and enter the
> calibration number for those settings. Then you would just draw
> straight segments across your tissue structure and use the "Analyze
> -> Measure" to get your measurement.
>
>
> Mark.
>
>> On Thu, 18 Aug 2005, Sharat Pankajavihar Sasi wrote:
>>
>> Hello,
>>       I am a new user of the Image J software and had few queries on the
>> application I am looking for. My issue is as stated below:
>>
>> "I have Calibration pictures that were taken at 40X lense and with different
>> zooms on the camera say 50, 77, 88 etc. These images taken need to be
>> calibrated from pixels to microns in the Image J software if possible. Then,
>> the stained images of the tissue taken with the same lens and zoom under the
>> same miscroscope need to be added in the software and the thickness and
>> diameter need to be measured in microns."
>>
>> Can this be done with Scion imaging software? Could you please
>> advice me on the
>> same? If its not possible can anyone please suggest me an alternative?
>>
>> Thanking You,
>>
>> Sharath. P. Sasi
>>
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>>
>

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