http://imagej.273.s1.nabble.com/measurement-within-outlined-area-tp3705036p3705037.html
The problem is that i'm not sure how to outline the outline :) You're right, inverting the image and then using the wand tool is a possibilty; then I have to invert it again, plus it doesn't give me a lot of flexibility in terms of what I can measure. I'll probably have to do it though unless I figure something else out..
> All these are possible, if you can select the outlined cell (create a
> roi around it). As for how to do this automatically, you could look at
> the wand tool, or perhaps analyze particles. I've had success with
> inverting an image so that white areas look like black particles, then
> using particle analyzer to get measurements on that area.
> dave
>
> -----Original Message-----
> From: ImageJ Interest Group [
[hidden email]] On Behalf Of
> ANDREI SDRULLA
> Sent: Monday, August 15, 2005 1:01 PM
> To:
[hidden email]
> Subject: measurement within outlined area
>
>
> Hi,
>
> I have z-stacks of 8 bit grayscale images that have structures (cells)
> outlined by hand (they're outlined by hand because thresholding
> doesn'twork well). I want to get measurements of the area
> outlined, mean pixel
> value, sum of all pixels within the outline, etc.. Any
> ideas/plugins on
> how to do this?
>
> Thanks in advance,
>
> Andrei
>