Re: Apply "Substract background" to all files in subfolders

Posted by Tony Collins-2 on
URL: http://imagej.273.s1.nabble.com/Apply-Substract-background-to-all-files-in-subfolders-tp3705174p3705190.html

It depends on how you wish to "subtract background". How does Fovea Pro
do it?

For uneven background you should use the menu command "Process/Subtract
Background". This "rolling ball algorithm" produces nice results but is
computationally intense and quite slow.

Other algorithms for BG subtraction use the mean of a user defined ROI
as a measure of BG. Although this may not be suitable for batch processing.

If you wish to merely subtract the minimum intensity in the image, then
you may need to write some macro code for this. Have a look at he
getStatistics() macro function.

Regards,

Tony

Dominik Geisel wrote:

> Thanks, but which option in Fovea Pro 3 would replace "subtract
> background" in ImageJ?

--
Tony Collins, Ph.D.
Facility Manager
Wright Cell Imaging Facility
Toronto Western Research Institute
13-407 McLaughlin Pavilion
399 Bathurst Street
Toronto, ON. M5T 2S8
tel. [phone] fax: [phone]
http://www.uhnresearch.ca/wcif

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