Problem with area measurement

Posted by kevin egan on Jul 19, 2005; 8:02pm
URL: http://imagej.273.s1.nabble.com/Problem-with-area-measurement-tp3705217.html

Hello again,

I have been having a problem with the Analyze particles tool in Image
J. If the area I am measuring has any fully enclosed white spaces
(after thresholding the image), it measures these areas as being part
of the  black areas. This skews our data as we wish to know the area
calculations for the black areas, not including any fully enclosed
white areas. I knonw this is probably a poor explanation so I made a
picture describing the problem. The picture is at my personal webspace
http://users.drew.edu/kegan/ex.jpg.
 I wish to use the Analyze particles tool to measure the area of the
black space in the picture, but when I measure it with Image J, it is
including these white "void" areas in the measurement. I have tried
limit to threshold, exclude on edges, flood fill, and various other
suggestions that I have found after searching through the archives.
Does the program currently have this functionality or is it in a
plugin that is out there or a plug in that is yet to be written?

Thank you very much for your help.

Kevin Egan