AND function

Posted by Florian Ulrich on Jul 14, 2005; 12:28pm
URL: http://imagej.273.s1.nabble.com/AND-function-tp3705233.html

Dear list members,

to find colocalization, I am comparing two 8-bit channels with the AND
function. I thought, it would calculate like

first channel value 128
second channel value 180
AND operation gives 128

or

first channel value 110
second channel value 55
AND operation gives 55

etc.

However, imagej sometimes does that, but also sometimes yields different
values for the AND function. How can that be? I am using imagej version
1.33u on 8-bit tif images gained from a Leica confocal microscope.

Help appreciated!

Best,

Florian Ulrich