Colocalization analysis using JACoP or Fiji

Posted by Aldo Vacaflores on
URL: http://imagej.273.s1.nabble.com/Colocalization-analysis-using-JACoP-or-Fiji-tp4999119.html

Hello,

I am looking at colocalization of two proteins using TIRF. I would like to obtain the Pearson coefficient and M1 & M2 coefficients of only a region of interest (ROI) between the two images. Can you select ROI’s to obtain the different coefficients using JACoP?
Also, I tried using Coloc2 which has the option to select an ROI's but I can’t figure out what value to use when it asks for the PSF.  Thanks for your help.
Aldo