Improving Particle Analyzer accuracy

Posted by Matthew Moore on
URL: http://imagej.273.s1.nabble.com/Improving-Particle-Analyzer-accuracy-tp4999626.html

Hi all,

I have a large number of images that I've created by splattering a small
blob of black ink on a needle onto a piece of plain white paper.  Once
the ink has dried I've been trying to use imagej to count the number of
blobs.

I've found the 'Particle Analyzer' function and created a small macro
that runs 'Make Binary' and then 'Particle Analyzer' on the images and
saves the count.

On low DPI images, saved straight to jpg, imagej consistently comes well
under manual counts.  On high DPI images, saved to tiff and then
converted (on max settings) to jpgs (so imagej can load them), imagej
consistently counts higher than the manual counts.

Does anyone have any ideas as to how I can improve accuracy?  I've spent
a while trying different pixel^2 and circularity settings.  I'm using
the version of imagej from the ubuntu repositories (1.44i).

Thanks in advance,

--
Matthew Moore
Surgical Materials Testing Laboratory
System Administrator
Telephone: [phone]
Email: [hidden email]

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