Posted by
Gabriel Landini on
URL: http://imagej.273.s1.nabble.com/Improving-Particle-Analyzer-accuracy-tp4999626p4999637.html
> The main problem is that the 'Make binary' that you have to use before
> running the particle analyzer introduces lots of noise into the image,
> so it picks up lots of false positives.
In that case you could process the image before thresholding or after.
Before: there are many filters that you can try: smoothing (mean, Gaussian),
median, morphological (greyscale closing/opening).
During: you could to try different methods for the actual thresholding step
(and IJ supports quite a few) or some other pixel classification.
After: binary closing/opening, filter by size, opening and closing by
reconstruction, median, etc.
You need to be aware that by any of that processing, you are changing the
original data.
> The dots are quite obvious really, it's a plain white
> piece of paper with black blobs on it.
I guess that probably the dots are not the problem, but the image or
background is not completely white, and the thresholding level that you are
using picks some of that intensity variation as "object".
Regards
Gabriel
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