calculating DF/F and movement correction
Posted by
Nicolás Pírez on
Aug 10, 2012; 2:28pm
URL: http://imagej.273.s1.nabble.com/calculating-DF-F-and-movement-correction-tp4999726.html
Dear List,
I am new at using ImageJ. I am using it to measure the changes in
fluorescence in a stack (time lapse). I have been using the ROIManager and
Multimeassure to obtain the fluorescence levels.
I have two questions: is there a function to calculate the DF/F
automatically? – eg: [(Fn/F0)/F0]X100%.
Additionally, I would like to be able to measure this value even in where
is some drift in the ROI across different slices in the stack (all images
are of the same field, but there is some movement X-Y). Is there a movement
correct function, so that the ROI can “follow” the movement?
Thanks a lot,
Nicolás Pírez.
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Nicolás Pírez, Ph.D.
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