Posted by
PEARSON Matthew on
URL: http://imagej.273.s1.nabble.com/filter-particles-tp4999791p4999793.html
Hi all,
I have some images of embryos captured using a colour camera
outputting RGB images. I have a batch of images of mouse embryos
captured in a petri dish without agar which gave the images a nice
black background almost darkfield in appearance. I then captured some
images later of different embryos but then the petri dish had agar in
and this has given a grey/blue background with the embryo not so well
contrasted and i want to put them all in a figure and have a uniform
background.
So in essence i want to change the background of the later images
(grey/blue) background to black without affecting the colour of the
embryo. I didn't think this would be a straight forward process on a
colour image. Does anyone have any suggestions? I have looked at the
background subtraction commands and this might be able to get me there
but thought this was more for fluorescence images and not sure its the
best way.. I also wondered about doing a colour threshold to identify
the embryo then setting the pixel value outside of the thresholded
embryo to zero if you can do that but i think this will will give a
jagged outline to the embryo.
Any ideas would be greatly appreciated.
Thanks,
Matt
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