Re: alignment with whole pixel shift

Posted by Stephan Saalfeld on
URL: http://imagej.273.s1.nabble.com/alignment-with-whole-pixel-shift-tp4999781p4999803.html

Hi Glen,

to avoid interpolation, just switch it off.  It does not matter whether
an alignment method estimates a sub-pixel translation if only it renders
the result by nearest-neighbor interpolation.  In Fiji's Register
Virtual Stack Slices and Linear Stack Alignment with SIFT, you can
uncheck `interpolation'.

Best,
Stephan





On Wed, 2012-08-15 at 11:37 -0700, Glen MacDonald wrote:

> Hello,
> Are there any plugins that will align a time-lapse stack with whole pixel shifts, thereby avoiding interpolation of intensities?   I'm needing to align some time-lapse images in order to measure intensities.  Sub-pixel precision is not necessary.  so far, searching the list server and plugin websites have not provided any answers.
>
> Thanks,
> glen
>
>
> Glen MacDonald
> Cellular Morphology Core
> Center for Human Development and Disability
> Box 357920
> University of Washington
> Seattle, WA 98195-7920  USA
> [phone]
> [hidden email]
>
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