http://imagej.273.s1.nabble.com/alignment-with-whole-pixel-shift-tp4999781p4999807.html
that does work. I had been mostly using StackReg, since it has a simple interface gave consistent results with prior datasets. But SIFT seems to align these time stacks well. I will need to become familiar with its parameters.
> Hi Glen,
>
> to avoid interpolation, just switch it off. It does not matter whether
> an alignment method estimates a sub-pixel translation if only it renders
> the result by nearest-neighbor interpolation. In Fiji's Register
> Virtual Stack Slices and Linear Stack Alignment with SIFT, you can
> uncheck `interpolation'.
>
> Best,
> Stephan
>
>
>
>
>
> On Wed, 2012-08-15 at 11:37 -0700, Glen MacDonald wrote:
>> Hello,
>> Are there any plugins that will align a time-lapse stack with whole pixel shifts, thereby avoiding interpolation of intensities? I'm needing to align some time-lapse images in order to measure intensities. Sub-pixel precision is not necessary. so far, searching the list server and plugin websites have not provided any answers.
>>
>> Thanks,
>> glen
>>
>>
>> Glen MacDonald
>> Cellular Morphology Core
>> Center for Human Development and Disability
>> Box 357920
>> University of Washington
>> Seattle, WA 98195-7920 USA
>> (206) 616-4156
>>
[hidden email]
>>
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