Question about Mice Axon Count using ImageJ

Posted by succinator on Sep 30, 2012; 2:18am
URL: http://imagej.273.s1.nabble.com/Question-about-Mice-Axon-Count-using-ImageJ-tp5000251.html

Hello, everyone. This is Steven. Right now I am working on quantification of axon in mice model. I am trying to figure out an efficient way to quantify my samples. I tried
1st:using threshold(black and white), in this step some of the axon detail was lost, some area is either too white or black, how could I avoid this, is there a way to locally treating the contrast
2nd:using nucleus count, this step was not really efficient, I am still trying to improve the count as it counts much less than what is out there
This is basically what I have done, I am willing to listen to any suggestions or other methods to quantify. Thanks, and I attached one of the image that I took to give an example to what I am working on.
http://postimage.org/image/z5jronpy9/