Re: Large number of particles
Posted by
Pål Baggethun on
Oct 10, 2012; 10:30am
URL: http://imagej.273.s1.nabble.com/Large-number-of-particles-tp5000357p5000359.html
Sascha,
In my experience the particle density in this image is too high. Not only do the particles overlap in a multitude of grayscales, they are also imaged at different magnifications due to the depth of field in your image (perhaps negligible, depending on your working distance). If possible, I suggest you disperse your sample considerably, for example with ultrasound in acetone, to get individual particles on a black background (e.g. a carbon tab). If successful you should be able to threshold or use watershed segmentation, or perhaps granulometric filtering, to extract your particle size/morphology.
P. Baggethun
Elkem Technology
Kristiansand, Norway
-----Original Message-----
From: ImageJ Interest Group [mailto:
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Sent: Wednesday, October 10, 2012 9:58 AM
To:
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Subject: Large number of particles
Hi folks,
I am trying to get the diameter of a large number of particles from a SEM image, but I failed so far.
Thersholding and "analyze particles" did not work. I tried to use "find edges" and take the result to extract the particles but my lack of knowledge with imageJ brought me to no result either.
Can somebody suggest a procedure to analyze the particles of the attached image?
Thanks in advance!
Sascha
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http://imagej.1557.n6.nabble.com/file/n5000357/1210A02032_crop.jpg>
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