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Re: Large number of particles

Posted by Adam Hughes on Oct 10, 2012; 3:58pm
URL: http://imagej.273.s1.nabble.com/Large-number-of-particles-tp5000357p5000371.html

I'm getting a file not found error.  Can you see if the link is still
working.  I'm doing extensive particle analysis right now and may be able
to help.  I agree with the above poster that if you can afford to redo the
imaging at sparse clustering, that's likely to be helpful.

On Wed, Oct 10, 2012 at 6:30 AM, Pål Baggethun <[hidden email]>wrote:

> Sascha,
> In my experience the particle density in this image is too high. Not only
> do the particles overlap in a multitude of grayscales, they are also imaged
> at different magnifications due to the depth of field in your image
> (perhaps negligible, depending on your working distance). If possible, I
> suggest you disperse your sample considerably, for example with ultrasound
> in acetone, to get individual particles on a black background (e.g. a
> carbon tab). If successful you should be able to threshold or use watershed
> segmentation, or perhaps granulometric filtering, to extract your particle
> size/morphology.
>
> P. Baggethun
> Elkem Technology
> Kristiansand, Norway
>
> -----Original Message-----
> From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
> superUke
> Sent: Wednesday, October 10, 2012 9:58 AM
> To: [hidden email]
> Subject: Large number of particles
>
> Hi folks,
>
> I am trying to get the diameter of a large number of particles from a SEM
> image, but I failed so far.
> Thersholding and "analyze particles" did not work. I tried to use "find
> edges" and take the result to extract the particles but my lack of
> knowledge with imageJ brought me to no result either.
> Can somebody suggest a procedure to analyze the particles of the attached
> image?
>
> Thanks in advance!
>
> Sascha
> <http://imagej.1557.n6.nabble.com/file/n5000357/1210A02032_crop.jpg>
>
>
>
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