Re: Creating individual tiles from one large image

Posted by ctrueden on
URL: http://imagej.273.s1.nabble.com/Creating-individual-tiles-from-one-large-image-tp5000374p5000386.html

Hi Jacqui,

> So what we need to do is divide the large image into separate tiles and
> output them as an image sequence.

You can also do this using the Bio-Formats command line tools:
   http://loci.wisc.edu/bio-formats/command-line-tools

You would use a series of calls to the "bfconvert" command line tool with
the "-crop" option to create tiles sized to your liking; e.g., "-crop
0,0,512,512", "-crop 512,0,512,512", etc., up to the size of your planes in
2 dimensions.

Regards,
Curtis


On Thu, Oct 11, 2012 at 12:22 AM, Jacqui Ross <[hidden email]>wrote:

> Hi All,
>
> We have a slide-scanner which images sequentially across a tissue section
> and then creates a large composite tiled image. We can output the files as
> individual tiles as well in RAW format.
>
> However, I am attempting to assist a few students who have deleted the
> individual tile files and now want to divide their images into individual
> tiles (e.g.512 x 512 pixels) for the analysis.
>
> So what we need to do is divide the large image into separate tiles and
> output them as an image sequence.
>
> I thought I could do it by creating rectangles and cropping or duplicating
> but can't work out how to get it working across the entire image. Can
> anyone help me with this?
>
> I'm starting to think it might be easier to rescan the sections and get
> the raw data again!
>
> Kind regards,
>
> Jacqui
>
> Jacqueline Ross
> Biomedical Imaging Microscopist
> Biomedical Imaging Research Unit
> School of Medical Sciences
> Faculty of Medical & Health Sciences
> The University of Auckland
> Private Bag 92019
> Auckland 1142, NEW ZEALAND
>
> Tel: [phone]
> Fax: [phone]
>
> http://www.fmhs.auckland.ac.nz/sms/biru/
>
>
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