Re: Large number of particles

Posted by Adam Hughes on
URL: http://imagej.273.s1.nabble.com/Large-number-of-particles-tp5000357p5000421.html

Well, if the clustering is that dense, it is a problem.  Depending on the
contrast at the boundaries, have you tried using the watershed filter in
ImageJ?  It may help to outline the boundaries of your particles better.

If that doesn't work, about how many clusters to free particles do you have
in the picture?  If you have, for example, 1000 clusters and 100 free
particles in your image, then you can run analyze particles with a
size/circularity cutoff to only sample the individual particles.  Do you
have enough to get a statistical sampling or just clusters?

On Mon, Oct 15, 2012 at 1:02 PM, superUke <[hidden email]> wrote:

> Hy Adam,
> yes. The link does not work anymore. It was basically an image of many
> particles clustered together. Due to the process, I cannot dilute them. The
> cluster look similar to this image:
>
> http://grmanet.sogang.ac.kr/media/results/animation/practical_anitsw/image/no_smoothing_click.jpg
> <
> http://grmanet.sogang.ac.kr/media/results/animation/practical_anitsw/image/no_smoothing_click.jpg
> >
> How do you do analysis of your images?
> Is there any way to analyze such images?
>
> Thanks.
>
> superUke
>
>
>
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