I need to copy and crop mutiple ROI from each image

Posted by Jasvinder Ahuja on Oct 22, 2012; 3:08pm
URL: http://imagej.273.s1.nabble.com/I-need-to-copy-and-crop-mutiple-ROI-from-each-image-tp5000475.html

Dear All,
I have pictures  with three channels and 12 sections. Each picture has few
DAPI stained nuclei. I want to make a plugin that copies the in focus
section of these individual nuclei as ROI and saves it to a new location. I
have no knowledge of JAVA.

Recently, I learnt VBA. So, if you could hint me towards the right
material, I want to give it a try. Any suggestions are welcome. It would be
great if I can do this in a batch mode.

So I need info on -
How images are stored?
How to determine (algorithm) the section with the image in focus?
Can it be different sections for each channel?
Specifically how a ROI can be selected and copied?
If this can be done in a batch mode?

I am a novice. There might be information regarding the same available. It
would be great if you could point me in the right direction.

I am delighted to see how this forum works. And would appreciate any
inputs/help

Jasvinder
Boerner Lab
Cleveland State University

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