Re: Licor Odyssey Western Blots

Posted by Tiago Ferreira-2 on Oct 23, 2012; 3:19pm
URL: http://imagej.273.s1.nabble.com/Licor-Odyssey-Western-Blots-tp5000485p5000491.html

On 2012.10.23, at 06:02 , Sieber, Anja wrote:
>
> Hi everyone,
> I have western blot pictures, either as a two channel picture or two separate pictures of the two channels (700/800nm). I want to analyze them, doing a density/ intensity plot over a sample lane. I am interested in the Intensity of the peaks (not necessarily the area, as there are often double peaks from not separated bands) and I want to know whether the two channels show peaks at the same position.
> My beloved output would be: xy data of the 700and800 channel in one step

The easiest way to obtain multiple line profiles on the same graph is to use the
Multi-Plot command.

1) Draw a line in channel 1 (or image 1), press "T" ("Edit>Selection>Add to Manager");
2) Move the c slider to channel 2 press "T" (or activate image 2 and press "Shift+E",
   "Edit>Selection>Restore selection")
3) In the ROI Manager, choose "Multi Plot" from the "more>>" dropdown menu.

HTH,
-tiago

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