Re: Quantifying Stained Retina

Posted by Rob van 't Hof-2 on
URL: http://imagej.273.s1.nabble.com/Quantifying-Stained-Retina-tp5000880p5000881.html

Hi Natalia,
A very usefull plugin for analysing stained specimens is Gabriel
Landini's colour deconvolution one
(http://www.dentistry.bham.ac.uk/landinig/software/cdeconv/cdeconv.html). This
is a very powerful plugin to separate different stains.

When you say "quantify" do mean mean measuring area covered with stain
or stain intensity (a contentious issue, see documentation to plugin).

You did not add an example image, so i don't know exactly what you want
to do. If you add an example image, people like me could create a basic
macro to show you how to do this kind of stuff.

bye,
Rob

On 19/11/2012 17:07, Natalia Chacon wrote:

> Hello everyone,
>
> I'm a new user of the ImageJ program.
> I'm currently working on a project where I have to quantify the amount of
> blue staining in an image.
>
> I've already found an article that is similar to what I'm doing, except its
> quantifying a red stain instead of a blue one.
> (Link provided here:
> http://rsbweb.nih.gov/ij/docs/examples/stained-sections/index.html)
>
> While this process will work from me, it seems I can't *Adjust>Threshold* so
> that I can quantify the blue!
>
> Please Advise.
> Thanks,
> Natalia
>
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> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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Dr. Rob van 't Hof
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Centre for Molecular Medicine
MRC IGMM
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United Kingdom

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