http://imagej.273.s1.nabble.com/Quantifying-Stained-Retina-tp5000880p5000887.html
I'm actually trying to measure blue. Its a dye that attaches to the iron in
> Hi,
> I can't see much blue in your sample, mostly pink/purple. is the material
> you want to measure the intensely stained pink roughly circular object that
> run diagonally (top left to bottom right) along the image?
> bye,
> rob
>
>
> On 19/11/2012 19:51, Natalia Chacon wrote:
>
>> On Mon, Nov 19, 2012 at 1:36 PM, Natalia Chacon
>> <
[hidden email]>**wrote:
>>
>> I mean to quantify the amount of blue staining. Now the staining
>>> correlates to the amount of iron in my sample.
>>> Here is an image of my sample.
>>>
>>>
>>> On Mon, Nov 19, 2012 at 11:33 AM, Rob van 't Hof <
>>>
[hidden email]> wrote:
>>>
>>> Hi Natalia,
>>>> A very usefull plugin for analysing stained specimens is Gabriel
>>>> Landini's colour deconvolution one (
http://www.dentistry.bham.ac.****>>>> uk/landinig/software/cdeconv/****cdeconv.html<
http://www.**>>>> dentistry.bham.ac.uk/landinig/**software/cdeconv/cdeconv.html<
http://www.dentistry.bham.ac.uk/landinig/software/cdeconv/cdeconv.html>
>>>> >**).
>>>>
>>>> This is a very powerful plugin to separate different stains.
>>>>
>>>> When you say "quantify" do mean mean measuring area covered with stain
>>>> or
>>>> stain intensity (a contentious issue, see documentation to plugin).
>>>>
>>>> You did not add an example image, so i don't know exactly what you want
>>>> to do. If you add an example image, people like me could create a basic
>>>> macro to show you how to do this kind of stuff.
>>>>
>>>> bye,
>>>> Rob
>>>>
>>>>
>>>> On 19/11/2012 17:07, Natalia Chacon wrote:
>>>>
>>>> Hello everyone,
>>>>>
>>>>> I'm a new user of the ImageJ program.
>>>>> I'm currently working on a project where I have to quantify the amount
>>>>> of
>>>>> blue staining in an image.
>>>>>
>>>>> I've already found an article that is similar to what I'm doing, except
>>>>> its
>>>>> quantifying a red stain instead of a blue one.
>>>>> (Link provided here:
>>>>>
http://rsbweb.nih.gov/ij/docs/****examples/stained-sections/****>>>>> index.html<
http://rsbweb.nih.gov/ij/docs/**examples/stained-sections/**index.html>
>>>>> <
http://rsbweb.nih.**gov/ij/docs/examples/stained-**>>>>> sections/index.html<
http://rsbweb.nih.gov/ij/docs/examples/stained-sections/index.html>
>>>>> >
>>>>>
>>>>> )
>>>>>
>>>>> While this process will work from me, it seems I can't
>>>>> *Adjust>Threshold* so
>>>>>
>>>>> that I can quantify the blue!
>>>>>
>>>>> Please Advise.
>>>>> Thanks,
>>>>> Natalia
>>>>>
>>>>> --
>>>>> ImageJ mailing list:
http://imagej.nih.gov/ij/list.****html<
http://imagej.nih.gov/ij/list.**html>
>>>>> <
http://imagej.nih.gov/**ij/list.html<
http://imagej.nih.gov/ij/list.html>
>>>>> >
>>>>>
>>>>>
>>>>> --
>>>> _____________________________
>>>> Dr. Rob van 't Hof
>>>> Reader
>>>>
>>>> Centre for Molecular Medicine
>>>> MRC IGMM
>>>> University of Edinburgh
>>>> Western General Hospital
>>>> Crewe Road, Edinburgh EH4 2XU
>>>> United Kingdom
>>>>
>>>> Phone: (+44)-131-6511031
>>>> email:
[hidden email]
>>>> _____________________________
>>>>
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>>>> >
>>>>
>>>>
>>> --
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>>
>
> --
> _____________________________
> Dr. Rob van 't Hof
> Reader
>
> Centre for Molecular Medicine
> MRC IGMM
> University of Edinburgh
> Western General Hospital
> Crewe Road, Edinburgh EH4 2XU
> United Kingdom
>
> Phone: (+44)-131-6511031
> email:
[hidden email]
> _____________________________
>
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>