Re: Quantifying Stained Retina

Posted by Gabriel Landini on
URL: http://imagej.273.s1.nabble.com/Quantifying-Stained-Retina-tp5000880p5000899.html

On Tuesday 20 Nov 2012 14:55:39 Natalia Chacon wrote:
> Rob, my mentor told me she used a stain called "Pearls Blue"
>
> Joel, I'm interested in quantifiying most of the stainings visible.
> (the background staining I don't desire to quantify.)
>
> What you're stating though does correlate with what my mentor has informed
> me. That the onl nuclei would be the most dense.  This would be due to the
> way the protien settles.

This is what Prussian, Pearls or Berlin blue looks like:

http://www.jichi.ac.jp/pathology/swfu/d/fe-10.jpeg

Your image looks like all contrast stain to me. So you might not have any iron
compounds there or it is so faint that the contrast stain prevails.

With Berlin blue, you can *demonstrate* that some iron compound is there, but
I am not sure if it can be used to quantify their quantity. Here it says it
cannot, so I would be careful how to interpret the intensity:

http://stainsfile.info/StainsFile/stain/pigment/perls.htm

Regards

Gabriel

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