Fw: measuring fluorescent intensity

Posted by nurul on Nov 21, 2012; 11:31am
URL: http://imagej.273.s1.nabble.com/Fw-measuring-fluorescent-intensity-tp5000911.html






   
          Tv79 - Copy.tif
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          Tv104 - Copy.tif
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Hi there,
 
I want to measure the fluorescence of dye in various part of eye. I'm attaching examples of my pictures. (Tv79, I want to measure intensity of whole picture, while in TV104, I want to measure selected structure only)
However, I am very confused using Image J. Should I take the int. Density measurement or mean gray area?
Is the measurement is affected by how large the area of cell/tissue? If so, this means I need to standardize area size in all my sample ( which is also another problem as I'm not able to do that..)
 
Can anyone care to guide me step by step way in measuring intensity for my case? (I'm currently only using technique as written in this blog : http://sciencetechblog.com/2011/05/24/measuring-cell-fluorescence-using-imagej/ )
 
If anyone can guide me, it is very helpful. Thank you everyone in advance

Dr. Nurul Alimah Abdul NasirMBBS (Malaya)Trainee Lecturer, Pharmacology, FOM UiTM+603-55211298+6013-2064397

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