http://imagej.273.s1.nabble.com/Thresholding-problem-tp5000916p5000918.html
> On Wednesday 21 Nov 2012 16:22:45 you wrote:
>> I use Objectcounter 3D to find objects in my image. As output I get an
>> image with all objects coded in a different intensity value (sample
>> picture attached). Now I want to threshold the image that I get only a
>> single object. If I do that using the threshold function of ImageJ
>> (adjusting upper and lower threshold to e.g. 265) I very often get 2
>> objects thresholded. Is that a bug or how can I threshold for only a
>> single intensity value?
> If you rimage has more than 256 levels, then the image histogram is mapped to
> 256, so I guess that two close values are binned to the same greyscale level.
> If you put the cursor on top of those objects thresholded at 265 you will see
> that one has grey level 264 and the other 265.
>
> To threshold them, set the threshold in the Thresholder applet with the "Set"
> button (eg min 264 and max 264) and then run the command "Make Binary". Only
> one of the two objects (the one painted in 264) is thresholded.
>
> Cheers
> Gabriel
>
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