Posted by
nurul on
Nov 22, 2012; 10:08am
URL: http://imagej.273.s1.nabble.com/measuring-fluorescent-intensity-tp5000912p5000927.html
Dear Michael,
The image that I captured was based on the standard exposure time. Some of the picture doesn't have that high saturation. (by the way, what is the actual meaning of large area that are saturated? - i assume it is the high level of intensity of the colour.)
Dr. Nurul Alimah Abdul Nasir
MBBS (Malaya)
Trainee Lecturer, Pharmacology, FOM UiTM
+603-55211298
+6013-2064397
________________________________
From: "Cammer, Michael" <
[hidden email]>
To: 'Nurul Alimah Abdul Nasir' <
[hidden email]>
Sent: Wednesday, 21 November 2012 11:26 PM
Subject: RE: measuring fluorescent intensity
Hi.
The images you sent have large areas that are saturated. Images need to be collected without saturated pixels to be properly analyzed.
Regards,
Michael
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Nurul Alimah Abdul Nasir
Sent: Wednesday, November 21, 2012 7:27 AM
To:
[hidden email]
Subject: measuring fluorescent intensity
Hi there,
I want to measure the fluorescence of dye in various part of eye. I'm attaching examples of my pictures. (Tv79, I want to measure intensity of whole picture, while in TV104, I want to measure selected structure only) However, I am very confused using Image J. Should I take the int. Density measurement or mean gray area?
Is
the measurement is affected by how large the area of cell/tissue? If so, this means I need to standardize area size in all my sample ( which is also another problem as I'm not able to do that..)
Can anyone
care to guide me step by step way in measuring intensity for my case?
(I'm currently only using technique as written in this blog :
http://sciencetechblog.com/2011/05/24/measuring-cell-fluorescence-using-imagej/ )
If anyone can guide me, it is very helpful. Thank you everyone in advance
Dr. Nurul Alimah Abdul Nasir
MBBS (Malaya)
Trainee Lecturer, Pharmacology, FOM UiTM
+603-55211298
+6013-2064397
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